Mary of Austria (b.1505-.1558) m Lajos II of Hungary and Bohemia (b.1506-.1526) in 1515
Anne (b.1521-.) m Zygmunt II Augustus, King of Poland (b. 1520-.) with issues
Jadwiga (b.1540-.) m Karl Christian, Elector Palatine (b.1537-.) with issues
Frederick III, Elector Palatine (b. 1560-.)
Dorothea (b. 1563-.)
Maria Christina (b. 1566-.)
Zygmunt III, king of Poland (b. 1542-.) m Anne of Austria (b.1549-.) with issues
Zygmunt IV, king of Poland (b. 1566-.)
Jadwiga (b. 1569-.)
Anna (b. 1572-.)
Elżbieta(b.1545-.) m John III, King of Sweden (b. 1537-.) with issues
John IIISigmund , King of Sweden (b. 1564-.)
Elizabeth (b. 1568-.)
Anna (b. 1572-.)
Sofia (b.1548-.) m Charles II, Duke of Lorraine (b.1543-.) with issues
Charles III, Duke of Lorraine (b.1564-.) m Catherine of Austria (b. 1562-.) with issues
Christina (b. 1566-.)
Maria Antonia (b. 1569-.)
Elizabeth (b. 1572-.)
Alessandro, Duke of Bari (b.1553-.) m Margherita Gonzaga (b.1564-.) with issues
Alessandro Sigomond, Duke of Bari (b. 1580-.)
Maria Eleonora (b. 1582-.)
Vladislaus ,III of Hungary and Bohemia (b.1523-.) m a)Elizabeth of Austria (b.1526-.1546) ,b)Mechthild of Bavaria (b.1532-.) in 1548 with issues
Lajos ,III of Hungary and Bohemia (b.1550-.) m Elizabeth of Austria (b.1554-.) with issues
Vladislaus ,IV of Hungary and Bohemia (b. 1572-.) m
Maria (b. 1574-.)
Maria (b.1552-.) m Ferdinand II, Holy Roman Emperor and Duke of Burgundy * (b. 1551-.) with issues
Maximilian III, Holy Roman Emperor (b. 1570-.)
Ferdinand Wenzel (b. 1574-1578)
Maria Anna (b. 1580-.)
István ,Duke of Slavonia(b.1555-.) m Sybil of Cleves (b.1557-.) with issues
Lajos, Duke of Slavonia (b. 1583-.)
Maria (b. 1585. 1589)
Anna Sibylla (b. 1592-.)
Anna (b.1558-.) m Karl Friedrich, Duke of Jülich-Cleves -Berg (b.1557-.) with issues
Wilhelm, Duke of Jülich-Cleves-Berg (b. 1578-.)
Maria Anna (b. 1581-.)
Mary (b.1526-.) m Albert V, Duke of Bavaria (b.1528-.) with issues
William V, Duke of Bavaria (b.1548-.) m Anna of Saxony (b. 1550-.) with issues
Maximilian I, Elector of Bavaria (b. 1570-.)
Maria Anna (b. 1573-.)
Ferdinand, Elector of Cologne (b. 1578-.)
Maria Anna (b. 1550-.) m Ferdinand II, Archduke of Tyrol (b. 1550-.) with issues
Archduke Leopold of Tyrol (b. 1567-.)
Maria Anna (b. 1571-.)
Christina (b. 1575-.)
John , Hereditary prince of Saxony (b.1498-1537) m Elizabeth of Hesse (b.1502-1557)
John George, Elector of Saxony (b.1522-.) m Anne of Austria (b.1528-.)
John George ,Elector of Saxony (b.1548-.) m Maria Eleanor of Cleves (b. 1551-.) with issues
John George, Elector of Saxony (b. 1570-.)
Anna (b. 1572-.)
Maria (b. 1574-.)
Sibylla (b. 1578-.)
Anna (b.1550-.) m William V, Duke of Bavaria (b.1548-.) with issues
Izabela of Poland (b.1519-.) m Francis III, Duke of Brittany (b.1518-1536)
Francis II Sigumond, King of France (b. 1536-.) m Margaret of Austria (b.1536-.) with issues
Francis III, King of France (b. 1555-.) m Mary of England (b. 1558-.) with issues
Charles Sigomond, Duke of Berry (b. 1558-.) m Mary of Scotland (b. 1557-.) with issues
Isabella (b. 1562-.) m Philip III of Spain (b. 1561-.) with issues
Sofia of Poland (b.1522-.) m Charles Victor of Brunswick-Lüneburg (1525–1553)
Charles Sigumond, Duke Brunswick-Lüneburg (b.1544–.) m Renata of Lorraine (b.1544-.) with issues
Charles Sigumond, Duke Brunswick-Lüneburg (b. 1568-.)
Christine (b. 1571-.)
Maria Renata(b. 1574-.)
Sophia (b.1548-.) m Charles, Archduke of Austria (b. 1540-.) with issues
Charles Sigumond, Archduke of Austria (b. 1571-.)
Anna (b. 1573-.)
Anna of Poland (b.1523-.) m John Albert I Mecklenburg (b.1525-.)
John VII, Duke of Mecklenburg-Schwerin (b.1548-.) m Elizabeth of Prussia (b.1551-.) with issues
John VIII, Duke of Mecklenburg-Schwerin (b.1570-.)
Catherine of Poland (b. 1526-.) m Archduke Ferdinand of Austria (b. 1529-.)
Archduke Ferdinand of Austria (b.1550-1601) m Maria Anna of bavaria (b. 1550-.)
Anna (b.1552-.1555)
Charles Siegmund (b.1556-.1562)
Catherine (b.1562-.) m Charles IV, Duke of Lorraine (b. 1564-.) with issues
Henry, Duke of Orleans (b. 1519-.) Catherine de' Medici, Countess of Boulogne (b. 1519-)
Louis, Duke of Orleans (b.1538-.) m Françoise de Bourbon, Duchess of Montpensirer (b. 1539-.) with issues
Madeleine (b.1540-.) m William IV, Landgrave of Hesse-Kassel (b.1532-.) with issues
*Charles V's original plan was to give Burgundy to his daughter Maria .
Henry VIII of England (d. 1524) married Catherine of Aragon (d. 1542)
Edward VI of England (b. 1516-.) m Maria of Portugal (b. 1521-.)
Catherine (b.1539-.) m James VI, King of Scotland (b. 1540-.) with issues
Henry IX, King of England (b. 1541-.) m Barbara of Austria (b.1539-.) with issues
Edward, Duke of Somerset (b. 1543-.) m Mary Fitzalan, Countess Arnold (b. 1540-.) with issues
Mary (b.1544-.) m Charles I, King of Portugal (b. 1545-.1582) with issues
Stillborn
Charles, Duke of Bedford (b.1551-.) m Elizabeth Manners (b.1557-.) with issues
Henry, Duke of York (b. 1518-.) m Catherine de Willoughby (b. 1519-.) with issues
Edward, Duke of York (b.1535-.) m Elizabeth Boleyn, Countess of Wiltshire (b. 1536-.) with issues
Catherine (b.1537-.) m Henry, Duke of Norfolk (b. 1535-.) with issues
Mary (b.1540-.) m Henry Gray, Marquess of Droste (b. 1537-.) with issues
Elizabeth (b.1544-.) , nun
John Archbishop of York (b. 1550-.)